Comparative analysis of CDR3 lengthdependent patterns in VHHs

VHHs, or nanobodies, are distinguished by their compact size, high stability, and unique ability to selectively target specific epitopes. The CDR3 region in VHHs, which plays a crucial role in antigen binding, exhibits significant diversity and varies among species. We systematically examined CDR3 length dependent patterns by analyzing NGS sequences from the PBMCs of Alpacas, Llamas and Bactrians, in conjunction with VHH structure data from the public database. Our findings will provide valuable insights forVHH engineering, synthetic library design, and the development of therapeutic nanobodies optimized for targeting diverse epitopes.