Gene expression profiles of primary myocardial cells treated with LPS and transfected siMETTL3 in rats

Methyltransferase-like 3 (METTL3) is the m6A methyltransferase which binds to METTL14 and wilms tumour 1-associated protein (WTAP) to form a complex catalyzing the m6A modification, the most abundant epitranscriptomic modification in eukaryotic mRNAs. And it has been demonstrated that m6A methylation mediated by METTL3 to be essential for inflammatory responses. To identify the messenger RNA (mRNA) profile in the primary myocardial cells that were treated with lipopolysaccharide (LPS) and/or small interference siMETTL3, six groups of primary myocardial cells were used for analysis. Overall design: All primary myocardial cells were derived from the same initial isolate (pooled from multiple rats). Primary cardiomyocytes were treated with small interference siMETTL3 or siNC for 24 h and then treated with LPS for 6 h.Three replicates of each condition were used for RNA extraction, RNAseq library preparation, and paired-end Illumina sequencing.