Abiotic stress induced “common-alarm signals” in plants

Thus, to interpret the involvement of ROS in stress signalling, the publicly available transcriptome datasets from selected AEM encoding genes of Arabidopsis thaliana including aox1a (mitochondria; (Umbach et al., 2005), cat2 (chloroplast, peroxisome and mitochondria; (Vanderauwera et al., 2005), sal1 (chloroplast and mitochondria; (Wilson et al., 2009), tapx (chloroplast; (Maruta et al., 2012), vtc1 (cytoplasm and nucleus; (Foyer et al., 2012; Pastori et al., 2003) and vtc2 (cytoplasm and nucleus; (Foyer et al., 2012) were utilized for analysis. There are atleast 50% overlap between the differentially expressed genes (DEGs) in Arabidopsis mutants including tapx, cat2, aox1a, vtc1 and vtc2 (Fig. 2A-B). The highest number of up- and down-regulated DEGs was observed in sal1 mutant, indicating it as one of the key regulators of nuclear gene expression. The other mutants follow the order of vtc2>tapx>vtc1>aox1>cat2, as per the total number of DEGs (Fig 2A). The median value indicated that the maximum change in gene expression in cat2, followed by sal1>tapx>vtc2>vtc1>aox1 and sal1>vtc1>vtc2>tapx>aox1 in case of up- and down-regulated genes, respectively (Fig. 2A). Thus, the maximum impact on nuclear gene expression was noticed under sal1 (as per DEGs number) and cat2 (as per expression change), which were incidentally having multiple sub-cellular localization.