CPD-seq mapping of UV damage repair in elf1 mutant yeast

Elf1 is an important transcription elongation factor that has been implicated in transcription coupled-nucleotide excision repair (TC-NER). Here, we have used a high-throughput sequencing method known as CPD-seq to map the repair of UV-induced cyclobutane pyrimidine dimers (CPDs) at single nucleotide resolution across the yeast genome in elf1 mutant cells. Analysis of CPD repair indicates that Elf1 is important for CPD repair in the transcribed strand (TS) of yeast genes, indicating it plays an important role in TC-NER.Elf1是一类重要的转录延伸因子，已被证实参与转录偶联核苷酸切除修复（transcription coupled-nucleotide excision repair, TC-NER）过程。本研究采用一种名为CPD-seq的高通量测序方法，在单核苷酸分辨率水平上，绘制了elf1突变酵母细胞全基因组范围内紫外线诱导的环丁烷嘧啶二聚体（cyclobutane pyrimidine dimers, CPDs）的修复图谱。对CPD修复情况的分析表明，Elf1对酵母基因转录链（transcribed strand, TS）上的CPD修复具有关键作用，这证实其在TC-NER过程中发挥着重要功能。