Endothelial cell infection and dysfunction, immune activation in severe COVID-19

SARS-CoV2 infects endothelial cells and induce the dysfunction of them. I. We infected WT C57BL/6J or K18 hACE2 transegnic mice in both male and female mice with 2x10^5 pfu SARS-CoV2. Then those mice were eutanized 4 days post challenge and the lungs were removed and processed for total RNA preparation. II. We infected female WT C57BL/6J or K18 hACE2 transgenic mice with 2x10^5 pfu SARS-CoV2. Then those mice were eutanized 4 days post challenge and the lungs were removed and processed for single cell suspensions.