Cerebellar Neuropixels recording and whisking tracking in mice during downregulation of cerebellar Golgi cells (v1)

This dataset originates from an experiment aiming to investigate neuronal representations in the lateral cerebellar cortex of whisking behaviour in mice during normal and reduced Golgi cell inhibition. The experiment involved concomitant Neuropixels I recording of lateral cerebellar cortical activity and high-speed camera recording of spontaneous whisking in head-fixed mice. Downregulation of Golgi cell inhibition was achieved after a baseline recording period via CNO-mediated activation of inhibitory DREADDs (designer receptors exclusively activated by designer drugs, hM4Di), selectively expressed by Golgi cells in this brain region.

The dataset includes electrophysiological, behavioural and imaging data obtained from 17 hM4Di-expressing/control (C57BL6J) mice who were administered CNO/phosphate buffered saline (PBS). Electrophysiological data are processed data that have undergone a spike-sorting step, necessary to identify clusters of spikes belonging to the same neuron from raw extracellular recording traces. Available whisking information consist in processed data informing about the position of user-specified whisker landmarks obtained from the raw video recordings. Imaging data include images from cerebellar coronal slices acquired with a confocal fluorescent microscope, used to determine the location of the recording and the expression of hM4Di receptors. Additionally, the dataset also includes Neuropixels recordings from three separate mice who were administered Muscimol to control for drug diffusion in the cerebellar cortex.