The large GTPase AtGBPL3 links nuclear envelope formation to lamina-associated gene silencing [RNA-seq]

Guanylate Binding Proteins (GBPs) are prominent regulators of immunity unknown to be required for nuclear envelope formation and function. The Arabidopsis GBP orthologue AtGBPL3 was identified as a novel component of the plant lamina, with essential functions in mitotic nuclear envelope reformation. AtGBPL3 is preferentially expressed in mitotically active root tips, accumulates at the nuclear envelope and interacts with further components of the nuclear lamina. To investigate effects of defective lamina components on gene expression, we analysed the transcriptomes of 4 day old wild-type, gbpl3-5, crwn4-1, crwn1-1crwn4-1, as well as gbpl3-5 mutants expressing GBPL3-GFP or the GTPase-dead GBPL3(K83A)-GFP under control of the AtGBPL3-promoter. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Arabidopsis thaliana (Col-0 and different mutants defective for putative components of the plant nuclear lamina (gbpl3-5, crwn4-1, crwn1-1crwn4-1), or transgenic lines (expressing either pAtGBPL3::GBPL3genomic-GFP or pAtGBPL3::GBPL3(K83A)genomic-GFP in the gbpl3-5 background)