Supplementary Figure 3. Elevated pS129 levels in cortical brain homogenates following inoculation of α-synuclein pre-formed fibrils in vivo.

Supplementary Figure 3. Elevated pS129 levels in cortical brain homogenates following inoculation of α-synuclein pre-formed fibrils in vivo. aSyn PFF-1-, PFF-2- and PBS- inoculated mice were unilaterally injected into the right dorsal striatum and cortical brain homogenates were collected at 16 weeks post injection for immunoblots. (A-C) The levels of pS129 and total human aSyn found in the detergent-soluble (RIPA) fractions from PFF-1 and PFF-2 samples were expressed as the percentage fold change relative to PBS samples (B-C). No significant differences were found between PFF-1 and PFF-2 when comparing pS129 (unpaired t-test, p>0.05) or human aSyn (unpaired t-test, p>0.05). (D-F) The levels of pS129 and total human aSyn found in the detergent-insoluble fractions (UREA solubilization of RIPA-insoluble pellets) were expressed in the same way as the detergent-soluble fractions (RIPA) (E-F). No significant differences were found between PFF-1 and PFF-2 when comparing pS129 (unpaired t-test, p>0.05) or human aSyn (unpaired t-test, p>0.05).