Mammalian circRNA Atlas

We developed a new pipeline for accurate circRNA detection. We build a new atlas for characterizing circRNA in human, monkey, mouse, rat and drosophila brain. Our approach allows full annotation of circRNAs with fewer false positives and negatives than any individual pipeline or combination of them. Moreover, our approach is more sensitive than any individual pipeline and allows more accurate quantification and larger number of differentially expressed circRNAs.我们开发了一套用于精准检测环状RNA (circRNA) 的全新分析流程。我们构建了一套全新的图谱，用于表征人、猴、小鼠、大鼠及果蝇大脑中的环状RNA。相较于任何单一分析流程或流程组合，本方法能够实现环状RNA的全面注释，且假阳性与假阴性率更低。此外，本方法的灵敏度优于所有单一分析流程，可实现更精准的定量分析，并能检测到更多数量的差异表达环状RNA。