Comparative results of HPV GA test, TaqMan uniplex qPCR and AllGlo quadruplex qPCR.

The agreement rate between AllGlo quadruplex fluorescence quantitative PCR, HPV GA test and TaqMan uniplex qPCR was 100%. Single-tube AllGlo probe quadruplex fluorescence quantitative PCR could simultaneously type HPV 6, 11, 16, and 18 and quantitate the viral load of each HPV at the same time. Compared with the HPV GA test and TaqMan uniplex qPCR method, the AllGlo quadruplex qPCR method enjoys a high sensitivity and a wide linear range. For the same sample, the positive rate and accuracy of the AllGlo quadruplex qPCR method was higher than those of the HPV GA test and TaqMan uniplex qPCR method. #Others:other HPV type,including HPV31,33,35,39,42,43,44,45,51,52,53,56,58,59,66,68,CP8304.