Electrophysiological and WB data

The hypothesis of this research is that repeated high-frequency visual stimulation (HFS), but not low-frequency visual stimulation (LFS), can lead to a LTP (long-term potentiation)-like increase of visually-evoked field potentials (VEP) in the primary visual cortex (V1), and this potentiation could be caused by more trafficking of membrane AMPA and NMDA receptors. To test this hypothesis, This study examined the effects of repeated HFS (9 Hz) versus LFS (1 Hz) visual stimulation on VEPs and the membrane protein content of AMPA / NMDA receptors in the V1 of cats. We found that repeated HFS caused a long-term improvement in peak-to-peak amplitude (N1P1, P1N2) of V1-cortical VEPs in response to visual stimuli at HFS-stimulated orientation (SO: 90°) and non-stimulated orientation (NSO: 180°), but the effect exhibited variations depending on stimulus orientation: the amplitude increase of VEPs in response to visual stimuli at SO was larger, reached a maximum earlier and lasted longer than at NSO. By contrast, repeated LFS had not significantly affected the amplitude of V1-cortical VEPs in response to visual stimuli at both SO and NSO. Furthermore, the membrane protein content of the key subunit GluA1 of AMPA receptors and main subunit NR1 of AMPA receptors in V1 cortex was significantly increased after HFS but not LFS when compared with that of control cats. These results indicate that HFS can induce LTP-like improvement of VEPs and an increase in membrane protein of AMPA and NMDA receptors in the V1 cortex of cats, which is similar to but less specific to stimulus orientation than the classic LTP.
The dataset folder includes two sub-folders: one is for Electrophysiological data, which shows repetitive measures of VEP amplitude in the V1 cortex of each cat (Cat1-4) before and at different time points (0-180 min with an interval of 15 min) after the end of HFS or LFS in response visual stimulus at stimulated orientation (SO) and non-stimulated orientation (NSO). Another sub-folder is for Western blot data, which shows the Western blot bands of the key subunit GluA1 of AMPA receptors and the main subunit NR1 of NMDA receptors as well as β-tubulin (internal reference) in the V1 cortex of 6 cats after HFS, 6 cats after LFS and 6 control cats (CTL). Statistical comparison of VEPs amplitude before and after repeated HFS or LFS as well as the normalized OD of GluA1 and NR1 in the V1 cortex between groups was done using ANOVA and LSD (least significance difference) Post hoc pairwise tests. More detailed descriptions for the electrophysiological data and Western blot data are included in each sub-folder.

本研究假设反复的高频视觉刺激（HFS）而非低频视觉刺激（LFS），能够导致初级视觉皮层（V1）中视觉诱发电位（VEP）的长期增强（LTP）样增加，而这种增强可能由膜上AMP A和NMDA受体的更多转运所致。为验证此假设，本研究检视了反复HFS（9 Hz）与LFS（1 Hz）视觉刺激对猫V1中VEP及膜蛋白含量（AMP A/NMDA受体）的影响。研究发现，反复HFS导致V1皮层VEP对HFS刺激方向（SO：90°）和非刺激方向（NSO：180°）视觉刺激的峰-峰值（N1P1，P1N2）幅度在HFS刺激后出现长期提升，但该效应随刺激方向的不同而呈现出差异：在SO处VEP幅度的增加更为显著，达到最大值的时间更早且持续时间更长；与此相对，反复LFS对SO和NSO两种刺激方向的V1皮层VEP幅度均未产生显著影响。此外，与对照猫相比，HFS显著增加了V1皮层中AMP A受体关键亚基GluA1和NMDA受体主要亚基NR1的膜蛋白含量，而LFS则没有。这些结果表明，HFS能够诱导VEP的LTP样改善及V1皮层中AMP A和NMDA受体膜蛋白含量的增加，这一效应与经典LTP相似，但在刺激方向特异性方面则略逊一筹。

数据集文件夹包含两个子文件夹：一个为电生理数据文件夹，展示了每只猫（Cat1-4）在HFS或LFS结束后不同时间点（0-180分钟，间隔15分钟）对刺激方向（SO）和非刺激方向（NSO）视觉刺激的VEP幅度重复测量；另一个为Western blot数据文件夹，展示了6只HFS后、6只LFS后和6只对照猫（CTL）V1皮层中AMP A受体关键亚基GluA1、NMDA受体主要亚基NR1以及β-微管蛋白（内部参照）的Western blot条带。通过方差分析（ANOVA）和最小显著性差异（LSD）事后配对检验，对反复HFS或LFS前后VEP幅度以及V1皮层中GluA1和NR1的标准化光密度（OD）进行了组间比较。每个子文件夹中均包含电生理数据和Western blot数据的更详细描述。