Gene expression changes in response to doxycyline-induced expression of proinsulin (C96Y)-GFP in an INS-1 insulinoma cell line

A doxycyline-inducible INS-1 insulinoma cell line expressing proinsulin (C96Y)-GFP was engineered. Addition of doxycyline causes the production of the proinsulin (C96Y)-GFP, which is retained in the endoplasmic reticulum. This study analyzes the gene expression changes that occur after doxycyline-induced expression of proinsulin (C96Y)-GFP for 24h, 48h and 5 days. Expression changes were compared between control un-induced cells and cells treated with doxycyline. Three replicates (experiments) were performed for each time point.本研究工程构建了可经多西环素(Doxycyline)诱导表达胰岛素原(C96Y)-绿色荧光蛋白(GFP)的INS-1胰岛素瘤细胞系。向该细胞系中添加多西环素，即可诱导产生胰岛素原(C96Y)-绿色荧光蛋白(GFP)，该蛋白会滞留于内质网(endoplasmic reticulum)内。本研究分析了经多西环素诱导表达胰岛素原(C96Y)-绿色荧光蛋白(GFP)持续24小时、48小时及5天后，细胞所发生的基因表达变化。研究对比了未诱导对照组细胞与多西环素处理组细胞之间的基因表达差异，每个时间点均设置3次重复实验。