Deep mutational scanning of CliM arrest peptide

Ribosome arrest peptides undergo programmed translational stalling in response to changes in the cellular environment to feedback-regulate gene expression. In this study, we have elucidated the physiological role and molecular mechanism of CliM, an arrest peptide recently identified in Clostridia. To comprehensively identify residues critical for translation arrest by Cd CliM, we performed deep mutational scanning (DMS).