Single-Molecule Data - Lokanathan Balaji et al. "DNA binding and bridging by human CtIP in the healthy and diseased states"

These timecourses and datasets correspond to the publication: Lokanathan-Balaji et al. “DNA binding and bridging by human CtIP in the healthy and diseased states” bioRxiv 2023 (https://doi.org/10.1101/2023.12.14.571649)----Fig4+5_Datasets.zip and Fig4+5_Timecourses.zip folders contain the datasets and the individual timecourses, respectively, for the conditions addressed in Main Figures 4 and 5:    - Bare DNA    - DNA + wild-type CtIP     - DNA + lambda CtIP     - DNA + R100W CtIP     - DNA + deltaC608 CtIP     - DNA + deltaC782 CtIP     - DNA + deltaN500 CtIP     - DNA + deltaN790 CtIP 

Fig6.zip and Fig7.zip folders contain confocal data corresponding to Main Figures 6 and 7, respectively:    - Main Figure 6 – diffusion traces of AF-CtIP and confocal scans    - Main Figure 7 – confocal scans with looped-DNA and confocal kymographs of co-diffusion experiments

Supp_FigS3_Datasets.zip folder contains the datasets for the conditions addressed in Supplementary Figure S3:    - DNA + 0 nM WT-CtIP    - DNA + 0.4 nM WT-CtIP    - DNA + 4 nM WT-CtIP    - DNA + 10 nM WT-CtIP    - DNA + 40 nM WT-CtIP

Supp_FigS6_Datasets.zip and Supp_FigS6_Timecourses.zip folders contain the datasets and the individual timecourses, respectively, for the conditions addressed in Supplementary Figure S6:    - fork-end branched DNA (forked) + wild-type CtIP    - fork-end branched DNA (forked) + lambda CtIP

Supp_FigS7.zip and Supp_FigS8.zip folders contain the photon counts for confocal data addressed in Supplementary Figure S7 and Supplementary Figure S8:    - Supplementary Figure S7 – diffusion traces of AF-CtIP    - Supplementary Figure S8 – co-diffusion experiments with labeled forked DNA fragments and CtIP