Hypoxia-inducible factor-1α mRNA expression in Pacific herring Clupea pallasii

A hypoxia-specific responsive gene, hypoxia-inducible factor-1α (HIF-1α), was identified and tested as a potential biomarker for hypoxia exposure in Pacific Herring Clupea pallasii.

Lab experiments:

'Threshold_data.CSV' HIF-1a expression data from Theshold experiment

'Timecourse_data.CSV' HIF-1a expression data from Time-Course experiment

'Mean_threshold_plot.CSV' Mean HIF-1a expression and SE from Threshold experiment for Fig. 5

'Mean_timecourse_plot.CSV' Mean HIF-1a expression and SE from Time-Course experiment for Fig. 6

'ANOVA_Threshold.R' code for two-way ANOVA and Fig.2

'ANOVA_Timecourse.R' code for two-way ANOVA and Fig.3

Field data:

'All_field_qPCR.CSV' is the HIF-1a expression from the field and binary data identifying significantly elevated levels of expression.

'GLM_Field.R' is the code for the generalized linear models for the binary field data.