Single-target high-throughput transcription analysis reveal high levels of alternative splicing present in the FPPS/GGPPS from Plasmodium falciparum. Plasmodium falciparum 3D7

Given the great importance of alternative splicing and other transcriptional phenomena in gene regulation and the generation of protein diversity, we have investigated the processing of the FPPS/GGPPS transcript in P. falciparum by high-throughput sequencing methods in four time-points along the intraerythrocytic cycle of P. falciparum. We have identified levels of transcript diversity an order of magnitude higher than previously observed in this organism, as well as a few stage-specific splicing events.