Structure of human protein kinase CK2 catalytic subunit (CK2alpha, CSNK2A1 gene product) in complex with the cyclic peptidomimetic compound 12 discovered by high-throughput screening

The Alpha/Beta subunit interface of CK2alpha and CK2alpha' (CSNK2A1 and CSNK2A2) offers the opportunity to design inhibitors that do not inhibit the acitity of protein kinase CK2 per se, but the assembly of the CK2 holoenzyme. Using a high-throughput screening conducted at the FMP (Berlin) we found compounds that effectively inhibit the holoenzyme assembly, partly even isoform-specific (compound 15 is specific for CK2alpha, CSNK2A1). These datasets belong to crystals with compound 12 with CK2alpha.