Transcriptome profiling of slr-2, C.elegans C2H2 Zn-finger. Caenorhabditis elegans

Our slr-2 dataset showed strong overrepresentation of genes previously identified in a serial analysis of gene expression (SAGE) intestinal library (McGhee et al., 2006) (p 75% of genes common both datasets showed expression changes in the same direction, although the common dataset contained an approximately equal mixture of up and downregulated genes. Furthermore, more than fifty genes common to the lin-35 and slr-2 datasets are known to have intestinal-associated functions. That some of these common intestinal genes were absent from the gut SAGE library could be due to differences in the developmental stage of the animals assayed (adults versus L1s) as well as experimental approaches (SAGE versus microarrays) Keywords: analysis of staged mutant worms Overall design: We compared transcriptome profiles of slr-2 mutants and control N2 worms at L1 stage stage. Each comparison was done in triplicate with independently grown and isolated animals. Data for N2 L1 stage were from our previous experiments (Series GSE6547) To achieve higher accuracy, RMA values were calculated on the basis of 21 microarray experiments (including data from Series GSE6547)