Gene expression profile of T cells from Isocitrate Dehydrogenase (IDH) stratified human gliomas at single cell level

The brain tumor immune microenvironment (TIME) continuously evolves during glioma progression, but only a limited view of a highly complex glioma associated immune contexture across isocitrate dehydrogenase mutation (IDH) classified gliomas is known. Herein, we present an unprecedentedly comprehensive view of T cells from brain TIME at single cell resolution, which served as part of our pan-cancer T cell atlas analysis. The glioma associated CD45+ leukocyte were subjected to Fluorescence-activated cell sorting (FACS) from cryopreserved single cells. The leukocyte enriched single cell suspensions were obtained from freshly resected tumors of IDH-mutant primary (IMP; n=4), IDH-mutant recurrent (IMR; n=4), IDH-wild type primary (IWP; n=3), or IDH-wild type recurrent (IWR; n=4) glioma patients and quasi-normal non-glioma brain (NGB; n=2) from an epilepsy patient and a dysembryoplastic neuroepithelial tumor. T cells were digitally isolated from the single-cell RNA sequencing data based on the canonical lineage marker. *** Raw data are not provided for this study. Raw data are protected by MD Anderson internal review board (IRB)-approved protocol numbers LAB03-0687, LAB04-0001 and 2012-0441 and Baylor College of Medicine IRB-approved protocol number H-13798 ***