RNA-Seq ACSS2 KD BA
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Cellular RNA was isolated using the RNeasy® Mini Kit (Qiagen, 74104). RNA quality was923analysed and quantified via the Bioanalyzer (Agilent). 200 ng of total RNA was used for library924preparation. Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New925England Biolabs GmbH, Germany) according to the manufacturer’s protocol. The quantity and926quality of the libraries were assessed by BioAnalyzer (Agilent). Generated libraries were927sequenced on an Illumina NovaSeq instrument with around 35 million reads per library (100928bp, paired-end).
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Zenodo创建时间：
2024-01-29
