Bay 61–3606 is a sensitizer of TRAIL-induced apoptosis.

(A) Assay plate where Bay 61–3606 was identified as a sensitizer of TRAIL. After pre-incubation of test compounds (5 μM), cells were exposed to TRAIL (50 ng/ml). Cell survival was measured as a percentage of the control sample. The arrow indicates Bay 61–3606 (5 μM). (B) MCF-7 cells were exposed to TRAIL (indicated concentrations) with or without Bay 61–3606 (2.5 μM) for 24 h (upper) and Bay 61–3606 (indicated concentrations) with or without TRAIL (50 ng/ml) for 24 h (lower). (C) After exposure to the agents for 12 h, MCF-7 cells were subjected to immunocytochemistry using an active Bak antibody. (D) Caspase activity in MCF-7 cells exposed to Bay 61–3606 (5 μM) with or without TRAIL (50 ng/ml) for 24 h. (E) DNA fragmentation in MCF-7 cells was treated as seen in (D). Values are the mean ± SE from three separate experiments performed in triplicate. Asterisks indicate significant differences compared with the control (* P <0.05, and *** P-values < 0.001).