High Yap and Mll1 promote a persistent regenerative cell state induced by Notch signaling and loss of p53. High Yap and Mll1 promote a persistent regenerative cell state induced by Notch signaling and loss of p53

Murine small intestinal crypts of six independent mice were isolated and cultured as 3D-Organoids. Expression of NICD and loss of p53 mutagenesis was induced by 24h treatment with 4OH-tamoxifen in three of the cultures, resulting in three mutant organoid lines. The other three organoid lines were not exposed to tamoxifen and served as controls. Of each organoid line a duplicate was taken resulting in six control and six mutant samples that were compared in the analysis.