Sample numbers for Dopamine Added Swimming Induced Paralysis (DA-SWIP) and sorting experiments under 6-hydroxydopamine (6-OHDA)

Caenorhabditis elegans (C. elegans) is an excellent model system to study neurodegenerative diseases, such as Parkinson’s disease, as it enables analysis of both neuron morphology and function in live animals. Multiple structural changes in neurons, such as cephalic dendrite morphological abnormalities, have been considered hallmarks of neurodegeneration in this model, but their relevance to changes in neuron function are not entirely clear. We sought to test whether hallmark morphological changes associated with chemically induced dopaminergic neuron degeneration, such as dendrite blebbing, breakage, and loss, are indicative of neuronal malfunction and result in changes in behavior. We adapted an established dopaminergic neuronal function assay by measuring paralysis in the presence of exogenous dopamine, which revealed clear differences between cat-2 dopamine deficient mutants, wildtype worms, and dat-1 dopamine abundant mutants. Next, we integrated an automated image processing algor..., , , # Sample numbers for Dopamine Added Swimming Induced Paralysis (DA-SWIP) and sorting experiments under 6-hydroxydopamine (6-OHDA)

[https://doi.org/10.5061/dryad.msbcc2g4t](https://doi.org/10.5061/dryad.msbcc2g4t)

The table AUDDIT_Sorting_Table1_(1).xlsx contains sample numbers for experiments in this manuscript.

This excel table includes four variables:

A) Experiment, which details the test under analysis

B) Group and Type, which relates to different treatment groups or genotypes

C and D) Here N relates to the number of items described in column D. For instance, line 2 (N=9, Type = Proportion) means that 9 replicates were assayed, and the readout for each experiment is proportion (i.e., fraction of animals paralyzed). In line 19, for example, N=276, Type=Dendrites, this means that a total of 276 dendrites were analyzed for this experiment.

E) Total animals. This column desribes how many animals were used in total to obtain the data for all experiments described in that line.
,