Growing Malaria Parasites at a Critical Shaking Speed Mimicking Physiological Flow Reveals New Phenotypes for Invasion Ligands, Supporting Information

This repository contains the dataset and analysis scripts associated with the upcoming publication titled Growing malaria parasites at a critical shaking speed that mimics physiological flow conditions reveals new phenotypes for EBA and RH invasion ligands. The repository includes a comprehensive collection of data and scripts used to generate all the plots, along with videos showing how the red blood cells behave in culture media for different shaking speeds in the different shaking vessels. The growth assay data used for this experiment was collected in four batches, labelled GA1 to 4:

GA1: compares the different knockout lines. 

GA2: compares different hematocrits. 

GA3: compares different growth vessels.

GA4: contains more repeats of lines from GA1.

This repository offers all necessary resources to replicate the findings, including the complete codebase, raw data, and graphical representations of results. Researchers are encouraged to explore the included notebooks and datasets for detailed insights. Movies S1 to S4 show a top and side view of each type of plate/plask used on an orbital shaker as the shaking speed is increased.