Analysis of heterodimeric transcription factor complex specificities

This dataset contains sequencing reads for DNA ligands selected using novel consecutive affinity-purification SELEX assay. Here we have used this CAP-SELEX method identify pairs of transcription factors (TFs) that bind cooperatively to DNA and to characterize the DNA sequences recognized by these complexes. Study includes also regular HT-SELEX experiments that have been run for validation of individual TF specificities, in these cases the field of the second TF name has been replaced with an text “htSELEX”. Filenames are composed of following fields that have been separated with underscore "_" symbols. 1) Name of the TF1 2) TF2 name 3) Selection cycle of the experiment 4) Experimental batch 5) “Barcode”, 6+3 bases that flank the 40N randomized region For example: GLI3_SOX17_3_AAA_TAAACA40NCAA Or TBX3_htSELEX_1_AR_TAAGCC40NAGT