Jennifer Bestman, Holly Cline (2011) CIL:37080, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #5 (hour 6) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged daily over 3 days and shows the development of the dendritic arbor and axon over time. This is a second image of this cell at this timepoint.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为来自白化非洲爪蟾(Cynops albolineatus)神经索中枢发育中的神经元第5号图像（时间点6小时）。该神经元每日进行成像，历时3天，展示了树突和轴突随时间推移的发育过程。此图像为该时间点该神经元的第二次成像。

在图像获取前约24小时，通过活体内电穿孔法将该细胞转染编码eGFP的质粒DNA。活体蝌蚪被麻醉，置于玻片下方，并通过其透明皮肤直接使用定制构建的双光子显微镜进行成像。