Genetic interactions of heterochronic mutants.

1All animals examined were homozygous for null alleles of the genes indicated and carry an integrated transgene wIs78(scm::GFP; ajm-1::GFP) to mark seam cells. All alleles are null.
2Seam cell counts were performed on L4 animals except where indicated.
3Alae formation was assessed in the early L4 stage.
4Strains carrying the let-7 mutation additionally contained a linked unc-3 mutant allele. They were grown at 15°C to limit constitutive dauer formation that results from the unc-3 mutation at higher temperatures in these backgrounds.
5Seam cell fusion with no alae formation was observed in the other 85% of animals.
SEM, standard error of the mean; ND, not determined.