Transcriptome comparison of pancreatic islets of Urocortin3 null islets and wild type controls

The peptide hormone Urocortin3 (Ucn3) is abundantly expressed by mature beta cells, yet its physiological role is unknown. Transcriptome data from Ucn3 null islets compared to wild type controls reveals selective depletion for delta cell markers and supports a role for Ucn3 as a trigger for somatostatin-mediated negative feedback. Isolated islets from Ucn3 knockout animals compared to wild type littermate controls. Islets from three indivual males were used for each genotype, lysed in Trizol for RNA isolation and library construction.肽激素尿皮质素3（Urocortin3，Ucn3）在成熟β细胞中大量表达，但其生理功能尚未明确。通过对比Ucn3敲除胰岛与野生型对照的转录组数据，可观察到δ细胞标志物出现选择性表达下调，证实Ucn3可作为生长抑素介导的负反馈触发因子。本数据集以Ucn3基因敲除小鼠与同窝野生型小鼠的分离胰岛为实验材料：每个基因型组使用3只独立雄性个体的胰岛，经Trizol裂解后完成RNA提取与文库构建。