Transcriptomic study with knock-in of PIK3CD in tumoral experimental mouse model

The isoform delta of class I Phosphoinositide 3-kinase (PI3Kdelta) is expressed primarily in hematopoietic cells, and its inhibitor, idelalisib, is approved for the treatment of leukemia and lymphoma. PI3Kdelta orients the apicobasal axis and regulates lumen formation through assembly of the extracellular matrix. PI3Kdelta overexpression in hepatocellular carcinoma (HCC) cells (Huh7) leads to profound changes in cell morphogenesis and is associated to the up-regulation of stem cell markers. With tumoral experimental mouse model, investigations on PI3Kdelta implication were performed after knock-in transfection RNA from tumors were used to perform transcriptome Clariom S mouse experiments in triplicates for three individual samples in each experimental condition: transfection with empty vector (AAV) and transfection with PIK3CD vector.