Two-step RNA sequencing identifies a tissue-specific landscape of sense/antisense transcription

We report the complexity of the polyadenylated transcriptome of the mouse intestinal tract assessed by two step RNA sequencing. Screening and verification of non-annotated transcriptional units by different RNA-Seq protocols.本研究报道了经两步法RNA测序（RNA sequencing）解析的小鼠肠道聚腺苷酸化转录组的复杂性，并通过不同的RNA测序（RNA-Seq）实验方案完成了未注释转录单元的筛选与验证。