Single-nuclei transcriptomic analysis of the subthalamic nucleus reveals different Pitx2- positive subpopulations
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE133953
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Single nuclei from the subthalamic nucleus from Pitx2-Cre mice (Skidmore et al.Mol. Cell. Neurosci. 37, 2008) crossed with mCherryTRAP reporter (Gt(ROSA)26Sor-mCherry-Rpl10a) (Hupe et al. Nucleic Acid Res. 2014) that was used to FACS sort Pitx2mCherry positive nuclei at postnatal day 28 to identify subpopulations. Sequencing libraries were prepared from FACS sorted individual nuclei with the Smart-Seq2 protocol (Picelli et al. Nature Methods 2013)
创建时间：
2020-07-13
