Df(h15q13)/+ mouse model reveals loss of astrocytes and synaptic-related changes of the excitatory and inhibitory circuits in the medial prefrontal cortex.

RNA sequencing is applied to medial preforntal cortex (mPFC) in Df(h15q13)/+ mouse model of the 15q13.3 hemizygous deletion. The overall goal is to identify the transcriptomic signature of the 15q13.3 deletion. RNA sequencing was performed in mPFC of 8-10 weeks male Df(h15q13)/+ mice (n=7) and wild type littermates (n=8) using BGISEQ-500 platform.本研究以携带15q13.3半合子缺失（15q13.3 hemizygous deletion）的Df(h15q13)/+小鼠模型为研究对象，针对其内侧前额叶皮层（medial prefrontal cortex, mPFC）开展RNA测序（RNA sequencing）。本研究的整体目标为鉴定15q13.3缺失的转录组特征（transcriptomic signature）。实验采用BGISEQ-500测序平台，对8-10周龄的雄性Df(h15q13)/+小鼠（n=7）及其野生型同窝仔鼠（n=8）的内侧前额叶皮层组织进行RNA测序。