Macrophage and MDA-MB-231 coculture and mitochondrial transfer

The goals of this study were to determine the effects of lateral transfer of mitochondria on recipient cell biology. We therefore collected cancer cells with and without macrophage mitochondria, and macrophages with and without cancer cell mitochondria, and analyzed transcriptomes by single-cell RNA-sequencing. Through our analysis, we found that cancer cells that received macrophage mitochondria have an enrichment of transcripts associated with cell cycle progression compared to cancer cells without macrophage mitochondria. We further validated these findings with wet lab experiments using cell proliferation markers. Overall design: MDA-MB-231 cells cultured with Human blood monocyte derived macrophages. n=1 blood donor; 2 technical replicates per conditions.