Ori-Finder 3

Ori-Finder 3, for the computational prediction of replication origins in S. cerevisiae at the genome-wide level based solely on DNA sequences. The ARS exhibiting both an AT-rich stretch and ARS consensus sequence element can be predicted at the single-nucleotide level. For the identified ARSs in the S. cerevisiae reference genome, 83 and 60% of the top 100 and top 300 predictions matched the known ARS records, respectively. Based on Ori-Finder 3, we subsequently built a database of the predicted ARSs identified in more than a hundred S. cerevisiae genomes. Consequently, we developed a user-friendly web server including the ARS prediction pipeline and the predicted ARSs database.Ori-Finder 3 可仅基于DNA序列，在全基因组水平上对酿酒酵母（S. cerevisiae）的复制起始位点进行计算预测。该工具可在单核苷酸水平上，预测同时包含AT富集区段与ARS共识序列元件的自主复制序列（ARS）。针对酿酒酵母参考基因组中已鉴定的自主复制序列，其前100条与前300条预测结果中，分别有83%与60%可匹配已知的ARS记录。基于Ori-Finder 3，我们后续构建了涵盖百余个酿酒酵母基因组中预测所得ARS的数据库。据此，我们开发了一款集成ARS预测流程与上述预测ARS数据库的友好型Web服务器。

搜集汇总

背景与挑战

背景概述

Ori-Finder 3是一个专门用于预测酿酒酵母复制起点的数据集，基于DNA序列和Z-curve方法，能够以高精度在全基因组水平识别自主复制序列，其预测结果与已知ARS记录有较高匹配率，例如前100预测匹配率达83%。