Simplified MLST scheme for direct typing of <i>Leptospira</i> human clinical samples

Leptospirosis is a globally distributed zoonosis. Epidemiological data are scarce and present major challenge because of the varied clinical presentations. Multilocus Sequence Typing has already proven to be a robust molecular typing method providing accurate results for strain characterization. We have adapted our MLST scheme by reducing the set of <i>loci to facilitate Leptospira</i> typing directly from human clinical samples. The application of this 3-locus scheme provides <i>Leptospira</i> species and allelic profiles of the samples retaining the power of discrimination of the whole scheme. Moreover, an approach to the serogroups was also achieved. Our results contribute to the epidemiological study of Leptospirosis, since the direct typing on clinical specimens could detect and update allelic variants and serogroups present in a region. The simplified scheme allowed at the same time to take advantage of limited genetic material available in clinical samples that may increase the sources of information for epidemiological monitoring.

钩端螺旋体病（Leptospirosis）是一种全球分布的人畜共患病。由于临床表现呈多样化特征，其流行病学数据较为匮乏，相关研究面临诸多挑战。多位点序列分型（Multilocus Sequence Typing）已被证实为一种稳健的分子分型方法，可为菌株鉴定提供精准可靠的结果。本研究对原有多位点序列分型（MLST）方案进行了优化，通过缩减基因座（loci）数量，实现了直接从人类临床样本中完成钩端螺旋体（Leptospira）分型的目标。采用该三基因座分型方案，可在保留全方案分辨能力的前提下，获取样本的钩端螺旋体菌种信息与等位基因谱。此外，该方案还可实现血清群（serogroup）的初步鉴定。本研究结果可为钩端螺旋体病的流行病学研究提供重要助力：直接对临床标本进行分型，能够检测并更新某一区域内存在的等位基因变异与血清群；简化后的分型方案同时可适配临床样本中有限的遗传物质，从而扩充流行病学监测的信息来源。

搜集汇总

背景与挑战

背景概述

该数据集提供了一个简化的多基因座序列分型（MLST）方案，用于直接从人类临床样本中对钩端螺旋体（Leptospira）进行分子分型。该方案将基因座减少至3个，简化了分型过程，同时保持了与完整方案相同的区分能力，并能识别血清群，旨在支持钩端螺旋体病的流行病学监测和研究。