Genome-wide analysis of E2F1 dependent gene expression in mouse testicular tissue

The effect of E2F1 loss on gene expression in testicular tissue was analyzed in 20-day old E2F1-/-, E2F1+/- mice. The hypothesis was that there would be clear changes in expression of genes related to cell cycle machinery. The results show that E2F1 has wider implications in gene expression dynamics in testicular cell types than merely controlling cell cycle related genes. Total RNA obtained from testis tissue of 20-day-old E2F1-/- (KO), E2F1+/- (HZ) and E2F1+/+ mice (WT).本研究针对20日龄的E2F1基因敲除纯合子（E2F1-/-）、杂合子（E2F1+/-）小鼠，分析了E2F1缺失对睾丸组织基因表达的影响。研究假设为：与细胞周期调控系统相关的基因表达将出现显著变化。结果显示，相较于仅调控细胞周期相关基因，E2F1对睾丸细胞类型中的基因表达动态具有更为广泛的调控作用。实验所用总RNA提取自20日龄E2F1-/-（KO，敲除纯合子）、E2F1+/-（HZ，杂合子）及E2F1+/+（WT，野生型）小鼠的睾丸组织。