Pneumocystis jirovecii msg genes repertoires

Characterization of the expressed and non-expressed repertoires of P. jirovecii MSG I in patients. The full-length MSG I genes linked, or not linked, to the single copy promoter will be amplified from patients samples using generic primers. The PCR products will be deep sequenced using high throughput circular consensus sequencing that generates long and highly accurate reads. We will compare the composition of the expressed and non-expressed repertoires of MSG I between patients from different geographical locations and with various underlying conditions. The results will help understanding the mechanisms of mutually exclusive expression, and thus the strategy of antigenic variation of the fungus.