Proteome of Penicillium funiculosum NCIM1228 and ∆Snf1 grown in glucose and Avicel in the presence as well as the absence of calcium

Penicillium funiculosum NCIM1228 and ∆Ampk were compared across calcium-limited and calcium-replete cellulosic conditions to assess changes within the proteome using quantitative mass spectrometry. These comparative proteomic data provide insights into cellular responses to produce and secrete cellulase during nutrient limitation, and the role of AMPK in cellulase secretion., Profiling of the total proteome of NCM1228 and ∆Ampk was performed under calcium-limited and calcium-replete cellulosic conditions. NCIM1228 and ∆Ampk were cultured in Mandel’s medium having cellulose, or cellulose+calcium at 28˚C (120rpm) (in triplicates)(3). 10mL mycelial culture was harvested after 48h, and the remaining culture was incubated for another 72h to harvest the secretome. The secretomes were examined for the desired phenotype by SDS-PAGE and enzyme assays(4, 5). Once the distinct phenotypes associated with the two strains under given conditions were ascertained, the whole cell proteome was extracted from 48h mycelial samples as previously described in Randhawa et al (2023), and quantified by the BCA method. To assess the variance during sample preparation and measurements, we added 1µg 13C and 15N labeled human Apolipoprotein (Apo-1) (Sigma) to 50µg sample before processing. The samples were alkylated and reduced before proteolytic digestion with trypsin (1µg/50µg of prot..., , # Title of Dataset: Proteome of Penicillium funiculosum NCIM1228 and ∆Snf1 grown in glucose and Avicel in the presence as well as the absence of Calcium.

---

Give a brief summary of dataset contents, contextualized in experimental procedures and results.

Sample1: NCIM1228 grown in Avicel, Sample 1
Sample2: NCIM1228 grown in Avicel, Sample 2
Sample3: NCIM1228 grown in Avicel, Sample 2
Sample4: NCIM1228 grown in Avicel with Calcium, Sample 1
Sample5: NCIM1228 grown in Avicel with Calcium, Sample 2
Sample6: NCIM1228 grown in Avicel with Calcium, Sample 3
Sample7: ∆Snf1 grown in Avicel, Sample 1
Sample8: ∆Snf1 grown in Avicel, Sample 2
Sample9: ∆Snf1 grown in Avicel, Sample 3
Sample10: ∆Snf1 grown in Avicel with calcium, Sample 1
Sample11: ∆Snf1 grown in Avicel with calcium, Sample 2
Sample12: ∆Snf1 grown in Avicel with calcium, Sample 3

## Description of the data and file structure

Fle Name:

1. Work flow with q-value.txt: includes the Work flow and steps used to process the samples ...