Neuronal regulation of myenteric interstitial cells of Cajal (ICC-MY) in the colon

Interstitial cells of Cajal (ICC) generate contractile patterns of colonic motility. We investigated innervation of ICC within the plane of the myenteric plexus (ICC-MY) in proximal colon using mice expressing GCaMP6f in ICC. ICC-MY generated localized Ca2+ transients that couple to activation of ANO1 channels, a Ca2+-activated Cl- conductance. ICC are electrically coupled to SMCs, so activation or suppression of currents in ICC affects excitability of SMCs. ICC-MY displayed tonic inhibition, as the neurotoxin, TTX, increased the frequency of Ca2+ transients. Tonic inhibition was mimicked by a nitric oxide donor, NONOate, and by a guanylate cyclase agonist (Bay 58-2667). In contrast, ODQ mimicked the effects of TTX, increasing Ca2+ transients. Carbachol (CCh) increased Ca2+ transients in ICC-MY, and these effects were mediated by M3 muscarinic receptors. Neostigmine also increased Ca2+ transients, suggesting there is tonic activation of enteric excitatory neurons in colonic muscles. Sub..., , # Data from: Neuronal regulation of myenteric interstitial cells of Cajal (ICC-MY) in the colon

Dataset DOI: [10.5061/dryad.31zcrjf0k](10.5061/dryad.31zcrjf0k)

## Description of the data and file structure

The file includes image datasets from ICC-MY cellular calcium events under control and pharmacological agents.

This directory contains subfolders related to different compounds used in experiments. Each folder contains data corresponding to a specific control subfolder and treatment subfolder. Below is a brief description of each subfolder:

### Files and variables

#### File: Bay58.zip

**Description:** Contains experimental data related to Bay-58-2667 and its effects on cellular activity.

  2 subfolders:

control and in the presence of Bay-58-2667

#### File: CCH.zip

**Description:** Includes data for Carbachol (CCH) affecting calcium signaling in ICC-MY.

  2 subfolders:

Control and in the presence of CCH

#### File: MEN-10376.zip

**Description:** Contains data for experime...,