UBR5 mediates chemoresistance in colorectal cancer by attenuating ferroptosis via Lys11 ubiquitin-dependent stabilization of Smad3-SLC7A11 signaling

To elucidate the mechanisms underlying UBR5 deficiency-enhanced cytotoxic effects of Oxaliplatin, we performed transcriptome profiling by RNA-seq to explore the signaling pathways affected by UBR5. Colorectal cancer cell SW1116 stably expressing shUBR5 or control shRNA were treated with DMSO or 25µM of oxaliplatin (Oxa) for 48hr. Afterward, cells were harvested for RNA preparation. Total RNA was extracted using TRIzol reagent and sequenced on Illumina Novaseq 6000 sequencing instrument (BGI, China). There were four experimental groups (shNC+DMSO, shNC+Oxa, shUBR5+DMSO, shUBR5+Oxa) with 3 replicates for each.