Phenotypically Anchored Transcriptomics of Diverse Agrichemicals Revealed Congruent Pathways and Unique Gene Expression Signatures

Transcriptomic data for 48 hour post-fertilization (hpf) wildtype zebrafish developmentally exposed to 45 different agrichemicals. Overall design: mRNA sequencing was performed on four biological replicates of the five collected for each treatment. Collections of 8 animals performed over 28 different days with a single day match control collected to form a global control dataset compared to treated samples.The samples underwent PrepX Robotic RNA PolyA enrichment combined with NEBNext UltraII Directional RNA Library Prep/Illumina Stranded mRNA library preparation. Samples were split between three P3 flow cells (1.2 billion reads per run) and a P2 (400 million reads per run), and samples were sequenced usii an Illumina NextSeq 2000 (100 bp single end) resulting in 20 million read counts for each sample.