Distribution of the systemically introduced mutations in leaves from the primary and lateral shoots of Nicotiana benthamiana plants inoculated with TRSV-SpCas9-NbPDS or its derivative

Amplicon sequencing results of NbPDSa and NbPDSb in leaves from the primary and lateral shoots of plants inoculated with ALSV-NbRDR6 and TRSV-SpCas9-NbPDS, in leaves from the primary and lateral shoots of plants inoculated with TRSV-SpCas9-NbPDS-16K with or without ALSV-NbRDR6 preinoculation, and in leaves from an uninoculated plant as a control.