Role and function of PAX5 in BCR-ABL1 driven pre-B cells

In order to investigate the function of PAX5 in ALL, we isolated bone marrow cells from C57Bl6 mice and transformed them with BCR-ABL1. In a second transduction the BCR-ABL1 driven pre-B cells were transformed either with PAX5-GFP or empty vector control (GFP) and subjected to gene expression analysis. Three days post transduction, total RNA of GFP positive sorted cells were extracted and subjected to gene expression analysis.