Additional file 1: Figure S1. of Micro-anatomical quantitative optical imaging: toward automated assessment of breast tissues

Illustration of methodology used to select MSER intensity parameters. The intensity parameters MaxVariation, MinDiversity, and Delta were selected by varying each parameter one at a time. The area fraction (AF) was calculated for each representative image after each iteration of MSER. Each intensity parameter was plotted as a function of AF. Values for the intensity parameters were selected based on which values correctly isolated nuclei from the representative images and which values led to the largest differences between tumor and benign images. Scale bar 100Â Îźm. (ZIP 140 kb)本图示展示了用于选取最大稳定极值区域（Maximally Stable Extremal Regions，MSER）强度参数的实验方法。本次研究通过单次逐一调整单个参数的策略，完成了MaxVariation、MinDiversity及Delta三个强度参数的筛选工作。在每一轮MSER迭代完成后，对每张代表性图像计算其面积分数（Area Fraction，AF）。随后将各强度参数与AF的对应关系绘制成函数曲线。最终强度参数的选取依据为：既能准确从代表性图像中分割出细胞核，又可使肿瘤图像与良性图像间的差异达到最大的参数值。比例尺：100 μm。（压缩包大小：140 kb）