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Heat shock chaperone HSPB1 mitigates poly-glycine-induced neurodegeneration via restoration of autophagic flux

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HEK293T cells were transfected with GFP-poly-G-TurboID or GFP-TurboID as a control. Following biotin labelling, the cells were lysed in RIPA buffer to separate the soluble and insoluble fractions. The insoluble proteins in the pellet containing poly-G aggregates were further lysed in the lysis buffer containing 8 M urea to ensure effective solubilization

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2025-01-20
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