Saccharomyces eubayanus strain:CBS12357 Genome sequencing

Two different libraries were prepared and sequenced using Illumina technology. The first sequence data set was a 100 cycle paired-end library with an expected insert size of 150bp. The overlapping read pairs data were merged into 3,293,656 single longer “pseudo” reads of 143bp ± 20.5, which represented a total amount of 471 Mb, and were subsequently assembled into 372 contigs with a size of 500 bp or longer resulting in a total sequence of 11.49 Mbp (Table 2). In a second assembly step, the 50-cycle mate pair library (17,521,927 pairs) with a 8-kb insert size representing 1.75 Gb was used to further structure the S. eubayanus CBS12357 strain genome sequence. Scaffolding enabled to step from 372 contigs to 76 scaffolds (Table 2) which led to a slightly larger haploid genome size of 11.9 Mb sequenced with a coverage depth of 185-fold. The obtained scaffolded genome sequence was annotated using the MAKER2 pipeline (Holt & Yandell, 2011) resulting in the annotation of 5238 ORFs.