Short-term depletion of nicotinic acetylcholine receptor subunits in Drosophila enterocytes

Bulk RNAseq from whole Drosophila melanogaster guts expressing either mCherry-RNAi (control) or RNAi against nicotinic acetylcholine receptor subunits β1 and β3 (nAchRβ1 and nAchRβ3) under control of a enterocyte-specific driver to assess their role in barrier function. NP1::Gal4, tubGa80ts was used as a driver to induce mCherry (Bloomington line BL 35785), nAchRβ1(VDRC line v33825, pruned) or AchRβ3 (VDRC line v42742, pruned), knockdown by RNAi in enterocytes. Knockdown was induced for 3 days by shifting adult mated female flies (5 days old) to the permissive temperature (29ºC). 4 replicates were included for each genotype, consisting of 25 dissected whole midguts guts each (crop, malpighian tubules and hindgut were removed).