Microarray expression data from the dissected MNTBs of FVB wild-type mice at P0, P1, P2, P3, P4, P6 and P14

The medial nucleus of the trapezoid body (MNTB) contains a nearly homogeneous population of neurons, which are innervated by large glutamatergic nerve terminals called calyces of Held (CH). Key steps in maturation of CHs and MNTB neurons, including CH growth and competition, occur very quickly for most cells between postnatal days (P)2 and P6. Therefore, we characterized genome-wide changes in this system, with dense temporal sampling during the first postnatal week. We identified 541 genes whose expression changed significantly between P0-6 and clustered them into eight groups based on temporal expression profiles. Candidate genes from each of the eight profile groups were validated in separate samples by qPCR. 33 total samples were analyzed with 4-5 replicates of each age at P0, P1, P2, P3, P4, P6, and P14.