Ni-Sp185/333 proteins isolated from immune activated sea urchins show a wide range of size and variable presence among animals.

1Vd, Vibrio diazotrophicus injection (104−106/ml wCF); Ec, E. coli injection (104 to 106/ml wCF); L, LPS injection (1–2 μg/ml wCF).2Protein isolation by nickel affinity followed the protocol recommended by CloneTech.3The percentage of Sp185/333 proteins of the total protein yield was estimated by densitometry of Western blots and corresponding protein gels; see Materials and Methods.4nd, not done; na, not applicable.Ni-Sp185/333 proteins isolated from immune activated sea urchins show a wide range of size and variable presence among animals.