Single-cell RNA sequencing of Medicago truncatula roots after exposure to Ensifer meliloti (formerly Sinorhizobium meliloti)

Single-cell RNA sequencing of Medicago truncatula roots was generated from nuclei using the 10X Genomics' Chromium technology. Roots were harvested and processed 24h after exposure to Ensifer meliloti (formerly Sinorhizobium meliloti). Two independent experimental replicates were analyzed. Each replicate consisted of nuclei extracted from a pool of ~ 150 root segments exposed to Ensifer meliloti for 24 hrs. Each replicate was sequenced two times.